vivo fluorescence imaging system  (Clinx Science)

Journal: bioRxiv

Article Title: Radiotherapy Enhancement by Gold Nanocluster-functionalized Nanoliposomes Using Polychromatic Orthovoltage X-ray Irradiation

doi: 10.64898/2025.12.04.692299

Figure Lengend Snippet: (A) Effect of sonication temperature on liposome size, (B) polydispersity index (PDI), and (C) AuDDT encapsulation efficiency across formulations with increasing AuDDT content, cold sonication (20 °C) is shown in blue and hot sonication (60 °C) in red. (D) Effect of 0.2 µm extrusion on liposome size, (E) PDI and (F) AuDDT encapsulation efficiency across formulations with increasing AuDDT content (0 mol% to 2 mol%), non-extruded is shown in mustard and extruded in black. (G) Quantification of AuDDT content by SWIR-fluorescence and (H) ICP-MS, comparison between theoretical value shown in black and experimental value in mustard. (I) Comparison of encapsulation efficiencies determined by SWIR-fluorescence shown in black and ICP-MS in mustard. For A-C, the data represents n = 5-80 from seven independent batches. For D-F, the data represents n=5-25 from four independent batches. From G-I, the data represents n=5-86 from 20 independent batches. Linear regression fits were applied on G and H.

Article Snippet: Organs were isolated and separately imaged using bioluminescence and fluorescence imaging (Li-Cor Pearl Trilogy) to quantify the distribution of BPD and BPD-PC.

Techniques: Sonication, Encapsulation, Fluorescence, Comparison

Journal: bioRxiv

Article Title: Radiotherapy Enhancement by Gold Nanocluster-functionalized Nanoliposomes Using Polychromatic Orthovoltage X-ray Irradiation

doi: 10.64898/2025.12.04.692299

Figure Lengend Snippet: (A) Frontal and side views of a representative mouse injected with Au+BPD LPs at the different time points. (B) Biodistribution of BPD-PC fluorescence on mice bearing orthotopic pancreatic tumors, 3h, 5h and 24h post-injection, fluorescence signal on ex-vivo organs 24h post-injection are highlighted. (C) Biodistribution of AuDDT SWIR-fluorescence on mice bearing orthotopic pancreatic tumors, 3h, 5h and 24h post-injection, fluorescence signal on ex-vivo organs 24h post-injection is highlighted. The data represents n=9.

Article Snippet: Organs were isolated and separately imaged using bioluminescence and fluorescence imaging (Li-Cor Pearl Trilogy) to quantify the distribution of BPD and BPD-PC.

Techniques: Injection, Fluorescence, Ex Vivo

Journal: bioRxiv

Article Title: Radiotherapy Enhancement by Gold Nanocluster-functionalized Nanoliposomes Using Polychromatic Orthovoltage X-ray Irradiation

doi: 10.64898/2025.12.04.692299

Figure Lengend Snippet: (A) ICP-MS quantification of Au content in spleen, liver and tumor, at 3h, 5h and 24h post liposome injection. (B) BPD fluorescence signal in spleen, liver and tumor, at 3h, 5h and 24h post liposome injection. AuDDT signal intensity in spleen, liver and tumor, at 3h, 5h and 24h post liposome injection. Data represents n=9 from three technical repetitions. (D) Elemental mapping of orthotopic PANC-1 tumor sections by LIBS for quantification of phosphorus (P) and gold (Au) on Au+BPD treated mice, depicted are tumor tissue, at 3h, 5h and 24h post liposome injection and representative H&E staining of the tissue slide. Scale bar, 2 mm.

Article Snippet: Organs were isolated and separately imaged using bioluminescence and fluorescence imaging (Li-Cor Pearl Trilogy) to quantify the distribution of BPD and BPD-PC.

Techniques: Injection, Fluorescence, Staining

Journal: Medical Principles and Practice

Article Title: Long-Lasting Suppression of Mechanical Allodynia in Mice by Intrathecal Administration of an Anti-Cell Adhesion Molecule 1 Antibody

doi: 10.1159/000547340

Figure Lengend Snippet: Live imaging of 3E1-injected intrathecally into mice. a Upper panel; X-ray transmission image of a mouse. The contrast agent was injected intrathecally between lumbar vertebrae L1 and L2 (indicated by an arrowhead). The scale on the lower border of the picture indicates the slice number of the CT scan. Lower panel; CT scan image. Three images numbered 64, 68, and 81 are shown. The contrast agent is visible in white. b Chicken-mouse chimeric 3E1 (cm3E1) and control mouse IgG1 (mIgG) labeled with ICG (15 μg each) were administered intrathecally to back-shaved mice at the L5/L6 intervertebral space (indicated by arrowheads in the leftmost panel). ICG fluorescence was detected at the indicated timepoints. d0, the immediately after injection; d1–d7 and d23, days 1 to 7 and 23 (d1 means 24 h after the injection). On d23, the mouse was skinned prior to imaging; the framed area is enlarged in the inset.

Article Snippet: ICG-labeled cm3E1 or control mIgG1 (15 μg, each) was administered intrathecally to the L5/L6 intervertebral space of mice whose backs had been shaved, and ICG fluorescence was detected at the indicated timepoints using the Pearl Trilogy Small Animal Fluorescence Imaging system (LI-COR, Tokyo, Japan).

Techniques: Imaging, Injection, Transmission Assay, Computed Tomography, Control, Labeling, Fluorescence